Abstract
Following the i. v. injection of [7[alpha]-3H]androstenolone-[35S]sulfate (3H/35S = 3.3) in a normal female subject, the fraction of steroid sulfatides in plasma (3H/35S = 2.6) yielded 4 fractions of steroid sulfates with a comparable H3/S35 ratio (1.3-2.4) upon repeated chromatography. From these fractions androst-5-ene-3[beta],16[alpha],17[beta]-triol, 3]beta],17[beta]-dihydroxy-androst-5-ene-16-one, 3[beta],16[alpha]-dihydroxy-androst-5-ene-17-one, androst-5-ene-3[beta],17[beta]-diol, 3[beta]-hydroxy-androst-5-ene-17-one, 3[alpha]-hydroxy-5[beta]-androstane-17-one, 3[alpha]-hydroxy-5[alpha]-androstane-17-one and androst-4-ene-3,17-dione could be isolated and tentatively identified. These findings suggest a direct metabolism of suloconjugated androstenolon to androsterone and 5[beta]-androstan-3[alpha]-ol-17-one with androstenedione enolsulfate as possible intermediate.

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