PLASMINOGEN ACTIVATORS AS MARKERS OF TUMOR COLONIZATION POTENTIAL

  • 1 January 1983
    • journal article
    • research article
    • Vol. 3  (4) , 243-248
Abstract
Cell suspensions from the R 3230 AC rat mammary adenocarcinoma, when injected i.v. into F344 rats, invariably produce multiple lung foci within 10 days. The colonization potential of cultures obtained from these foci, and from cell populations exposed to 100 .mu.g/ml medium of both concanavalin A and wheat germ agglutinin for 5 passages was compared with the original cell line. Plasminogen activator activity (PAA) was determined in all 3 cell subpopulations, using S2251 (KABI) as chromogenic substrate. All cell lines retained their ability to grow after subcutaneous implant. The lectin resistant variant lost its capacity to nidate in the lung completely and also had the lowest PAA. In contrast, the cell population derived from the lung foci ranked highest in PAA.