An enzyme from rat liver catalysing conjugations with glutathione

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Abstract
An enzyme which catalyzes the formation of S-(2-chloro-4-nitrophenyl)glutathione from 3,4-dichloronitrobenzene has beer partially purified from the soluble fraction of rat liver. S-(2-Chloro-4-nitrophenyl)glutathione is converted into S-(2-chloro-4-nitrophenyl)-L-cysteine by rat-kidney homo-genate, and this compound can be acetylated to the mercapturic acid, N-acetyl-S-(2-chloro-4-nitrophenyl)-L-cysteine by rat-liver.slices. The soluble liver fraction can catalyze the formation of glutathione conjugates from a number of other mercapturic acid precursors. With bromobenzene, naphthalene, 1-chloronaphthalene and phenanthrene, rat-liver mlcrosomes and reduced trlphosphopyridine nucleotide as well as the soluble liver fraction are necessary in the formation of the glutathione conjugates. Glutathione cannot be replaced by oxidized glutathione, cyst-eine or N-acetylcysteine in these systems.