A micro method for separating free and esterified vitamin A

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Abstract
A micro method using Al2O3 as adsorbent was devised for separating vit. A alcohol from the esterified form. Under the conditions of the method, vit. A ester in light petroleum passed through the column, whereas vit. A alcohol was adsorbed and could be eluted with 20% acetone in light petroleum. The separation proved to be satisfactory either for artificial mixtures or biological extracts fortified with vit. A alcohol or ester.

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