EFFECTS OF 2-DEOXYGLtJCOSE, PHLORIZIN, INSULIN AND OUABAIN ON GLUCOSE-DEPENDENT ANAPHYLACTIC HISTAMINE RELEASE FROM GUINEA-PIG LUNG

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Abstract
R Under Oxygen in a substrate-free medium, anaphylactic histamine release from guinea-pig lung tissue was markedly enhanced by the addition of succinate but the histamine release from rat lung tissue was not increased. Succinate evidently increased O2 uptake irrespective of whether enhancing the histamine release or not. By glucose an increase in anaphylactic histamine release was produced in both species, but this was not accompanied by any significant increase in oxygen uptake in either species. Glucose reversed the anoxic inhibition of anaphylactic histamine release from guinea-pig lung tissue. This glucose-dependent histamine release was inhibited by 2 -deoxyglucose as well as phlor i-zin, and accelerated by insulin. Insulin removed the inhibition by phlorizin of the histamine release. Both 2 -deoxyglucose and phlorizin showed no effect on respiration, but the former partially inhibited aerobic histamine release. Ouabain inhibited the glucose-dependent anoxic histamine release in the same order of concentrations at which it inhibits active ion transport. But, this inhibitory action is not solely directed to the effect on glucose transport mechanism, because it also inhibited the aerobic histamine release occurring in the absence of glucose.