Studies on the Chemical Composition of Lipopolysaccharide from Neisseria meningitidis Group B

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Abstract
A lipopolysaccharide was isolated from N. meningitidis group B by phenol/water extraction and purified by differential ultracentrifugation. The preparation exhibited endotoxic properties as shown by the Limulus-lysate assay. Mild acid hydrolysis of the lipopolysaccharide yielded a lipid A fraction and a polysaccharide fraction. The lipid A fraction contained fatty acids, P and glucosamine. Analysis of the polysaccharide fraction revealed the presence of glucose, galactose, glucosamine, 2-keto-3-deoxyoctonic acid and P. There was no heptose.