The Hypoosmotic Swelling Test: An Update
- 1 January 1992
- journal article
- research article
- Published by Taylor & Francis in Archives of Andrology
- Vol. 29 (2) , 105-116
- https://doi.org/10.3109/01485019208987714
Abstract
The hypoosmotic swelling (HOS) test is a relatively new assay used to evaluate the functional integrity of the sperm's plasma membrane. In fact, more studies have been published on the applicability of the HOS test than any other new sperm indicator. The assay is based on the fact that fluid transport occurs across an intact cell membrane under hypoosmotic conditions until equilibrium is reached. Due to the influx of fluid, the cell will expand and bulge, especially in the tail, and this change can be readily observed with a phase contrast microscope. Earlier studies have yielded some confusion regarding the interpretation of the data. This review is an attempt to clarify and update the usefulness of the HOS test as a tool to evaluate the sperm function.Keywords
This publication has 39 references indexed in Scilit:
- Hypoosmotic swelling test in normo-, oligo-, astheno- and oligoasthenozoospermic men before and after swim-up separation of spermatozoaAndrologia, 2009
- The hypoosmotic swelling test as a useful adjunct to the semen analysis to predict fertility potentialFertility and Sterility, 1989
- The hypo-osmotic swelling test and the sperm mucus penetration test in determining fertilization of the human oocyteHuman Reproduction, 1989
- Evaluation of Computerized Analysis of Sperm Movement Characteristics and Differential Sperm Tail Swelling Patterns in Predicting Human Sperm In Vitro Fertilizing CapacityJournal of Andrology, 1989
- Hypoosmotic Swelling Test in the Prediction of Male FertilityArchives of Andrology, 1989
- Multivariate discriminate analysis of the relationship between the hypo‐osmotic swelling test and the in‐vitro fertilizing capacity of human spermInternational Journal of Andrology, 1988
- Correlation of Semen Analysis and Hypoosmotic Swelling Test with Subsequent PregnanciesArchives of Andrology, 1988
- Evaluating Glass, Polystyrene, and Polypropylene Containers for Semen Collection and Sperm WashingArchives of Andrology, 1988
- Improvement in the Quality and Fertilization Potential of a Human Sperm Population Using the Rise TechniqueJournal of Andrology, 1987
- The relationship between the human sperm hypoosmotic swelling test, routine semen analysis, and the human sperm zona-free hamster ovum penetration assayFertility and Sterility, 1985