Study of heat shock response of human umbilical vein endothelial cells (HUVECs) using cDNA microarray
- 1 January 2007
- journal article
- research article
- Published by Taylor & Francis in International Journal of Hyperthermia
- Vol. 23 (3) , 225-258
- https://doi.org/10.1080/02656730701295441
Abstract
Genetic response of human umbilical vein cells (HUVECs) to heat shock was studied using gene expression analysis. HUVECs were subjected to heat treatment at 44 degrees C and 55 degrees C for 1 h, respectively. Four hours after the treatment, gene expression of the treated cells and control cells (37 degrees C) were profiled using cDNA microarray. Data were analyzed using bioinformatics tools, and the results were verified by real-time quantitative PCR experiment. It has been shown that a large number of genes were regulated indicating global response to heat treatment at the genetic level.Keywords
This publication has 26 references indexed in Scilit:
- Change of individual vascular endothelial calcium during hyperthermiaJournal of Thermal Biology, 2006
- Study of non-uniform nanoparticle liposome extravasation in tumourInternational Journal of Hyperthermia, 2005
- Analysis of gene expression in apoptosis of human lymphoma U937 cells induced by heat shock and the effects of α-phenyl N-tert-butylnitrone (PBN) and its derivativesApoptosis, 2005
- Cellular effects of hyperthermia: relevance to the minimum dose for thermal damageInternational Journal of Hyperthermia, 2003
- The cellular and molecular basis of hyperthermiaCritical Reviews in Oncology/Hematology, 2002
- AP-1 as a regulator of cell life and deathNature Cell Biology, 2002
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001
- Implication of a Small GTPase Rac1 in the Activation of c-Jun N-terminal Kinase and Heat Shock Factor in Response to Heat ShockJournal of Biological Chemistry, 2001
- Role of c-jun Expression Increased by Heat Shock- and Ceramide-activated Caspase-3 in HL-60 Cell ApoptosisJournal of Biological Chemistry, 2000
- Mechanisms of calcium transport in human endothelial cells subjected to hyperthermiaJournal of Thermal Biology, 1999