Localization of Frog Virus Multiplication in Chick Embryo Cells by Immunofluorescence.

Publisher Website
Google Scholar
Abstract
Chick embryo cells infected with frog viruses No. 1 and 3 were fixed at various times after infection and stained with rabbit antibody labeled with fluorescein isothiocyanate. The data show the following: Viral antigens accumulate beginning about 8 hr. after infection in discrete cytoplasmic structures which increase in size and become altered with time. Frog viruses No. 1 and 3 cannot be differentiated by immunofluorescence with respect to siet of antigen accumulation and with respect to reactivity with heterologous antibody. Frog viruses differ from herpes simplex with respect to antigenic determinant sites and with respect to pattern of intracellular antigen accumulation.