Preparative separation of the complementary strands of DNA restriction fragments by alkaline RPC-5 chromatography
- 1 January 1978
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 5 (1) , 13-21
- https://doi.org/10.1093/nar/5.1.13
Abstract
High pressure reversed phase chromatography (RPC-5) at pH 12 was used for preparative separation of the complementary strands of the smaller DNA fragments which are generated by the Hae III restriction endonuclease digestion of Col El DNA. A single high pressure RPC-5 chromatographic step at neutral pH served to purify duplex fragments 70, 172, 250 and 440 base pairs long; each of these yielded two elution peaks upon chromatography under alkaline denaturing conditions.Keywords
This publication has 17 references indexed in Scilit:
- The nucleotide sequence surrounding the origin of DNA replication of Col E1Nucleic Acids Research, 1977
- Origin of replication of colicin E1 plasmid DNA.Proceedings of the National Academy of Sciences, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- Separation of the complementary strands of DNA fragments on polyacrylamide gelsNucleic Acids Research, 1977
- The Role of DNA Structure in Genetic RegulatioCRC Critical Reviews in Biochemistry, 1977
- Preparative fractionation of DNA restriction fragments by reversed phase column chromatography.Proceedings of the National Academy of Sciences, 1976
- Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresisBiochemistry, 1975
- The isolation and sequence analysis of transfer RNA The use of plaskon chromatography (RPC-5)Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Anion-exchange chromatography on reversed-phase columns Isolation and assay of nucleosides, nucleotides, and oligonucleotides from nucleic acids and from cytoplasmBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.1972