Bacterial actin: architecture of the ParMRC plasmid DNA partitioning complex

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Abstract
The R1 plasmid employs ATP‐driven polymerisation of the actin‐like protein ParM to move newly replicated DNA to opposite poles of a bacterial cell. This process is essential for ensuring accurate segregation of the low‐copy number plasmid and is the best characterised example of DNA partitioning in prokaryotes. In vivo , ParM only forms long filaments when capped at both ends by attachment to a centromere‐like region parC , through a small DNA‐binding protein ParR. Here, we present biochemical and electron microscopy data leading to a model for the mechanism by which ParR– parC complexes bind and stabilise elongating ParM filaments. We propose that the open ring formed by oligomeric ParR dimers with parC DNA wrapped around acts as a rigid clamp, which holds the end of elongating ParM filaments while allowing entry of new ATP‐bound monomers. We propose a processive mechanism by which cycles of ATP hydrolysis in polymerising ParM drives movement of ParR‐bound parC DNA. Importantly, our model predicts that each pair of plasmids will be driven apart in the cell by just a single double helical ParM filament.