A sensitive radiochemical assay for angiotensin-converting enzyme (kinase II)
- 1 July 1975
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 149 (1) , 297-300
- https://doi.org/10.1042/bj1490297
Abstract
[[125I]Tyr8]Bradykinin is degraded by angiotensin-converting enzyme to [125I]Tyr-Arg. The reaction product can be separated completely and recovered nearly quantitatively from unchanged substrate by cation-exchange chromatography. Thus it is possible to use [[125I]Tyr8]bradykinin at high specific radioactivity (about 400Ci/mmol) to measure the small quantities of angiotensin-converting enzyme encountered in small-scale cultures of pulmonary endothelial cells.Keywords
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