Gene transfer in intact animals
- 1 April 1980
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 284 (5755) , 422-425
- https://doi.org/10.1038/284422a0
Abstract
Resistance to methotrexate was induced in bone marrow cells of mice by transformation in vitro with DNA from a drug-resistant cell line. Transformed cells were injected in vivo and haematopoietic cells expressing resistance were selected by drug treatment of recipients. Transformed cells had elevated levels of dihydrofolate reductase and demonstrated a proliferative advantage over untransformed cells, indicating successful gene transfer.Keywords
This publication has 17 references indexed in Scilit:
- Gene Amplification and Drug Resistance in Cultured Murine CellsScience, 1978
- A rapid radiometric assay for dihydrofolate reductaseAnalytical Biochemistry, 1978
- Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donorCell, 1978
- The transfer and stable integration of the HSV thymidine kinase gene into mouse cellsCell, 1978
- Biochemical transformation of mouse cells by fragments of herpes simplex virus DNACell, 1977
- Transfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by purified herpes simplex viral DNA.Proceedings of the National Academy of Sciences, 1977
- Microcell-mediated transfer of murine chromosomes into mouse, Chinese hamster, and human somatic cells.Proceedings of the National Academy of Sciences, 1977
- Increase of dihydrofolate reductase activity in cultured mammalian cells after exposure to methotrexate.Proceedings of the National Academy of Sciences, 1967
- Inhibition of Dihydrofolic Reductase by Aminopterin and Amethopterin.Experimental Biology and Medicine, 1958
- STUDIES ON THE CHEMICAL NATURE OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPESThe Journal of Experimental Medicine, 1944