RNA Polymerase Activity Associated with Bacteriophage 6 Nucleocapsid
- 1 May 1979
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 43 (2) , 299-307
- https://doi.org/10.1099/0022-1317-43-2-299
Abstract
The Pseudomonas phaseolicola bacteriophage φ6 nucleocapsid incorporated ATP, UTP, CTP and GTP into ssRNA products which sedimented at the same rate as heat-denatured small and medium dsRNA components. Hybridization of the separated ssRNA products with denatured dsRNA revealed that the small ssRNA hybridized only to small dsRNA and the medium ssRNA to the medium dsRNA. Synthesis of the large ssRNA was not observed. The RNA polymerase reaction proceeded through a replicative intermediate-like RNA probably via a semiconservative mechanism.This publication has 3 references indexed in Scilit:
- Novel template requirements of N4 virion RNA polymerase.Proceedings of the National Academy of Sciences, 1978
- Virion-associated RNA polymerase required for bacteriophage N4 development.Proceedings of the National Academy of Sciences, 1977
- Synthesis of Bacteriophage 6 Double-stranded Ribonucleic AcidJournal of General Virology, 1976