A New Method for the Assessment of Phototoxic and Photoallergic Potentials by Topical Applications in the Albino Guinea Pig

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Abstract
To complete a previous publication on the assessment of phototoxicity reactions in the guinea pig, a new method is described which determines, in the same animals, the phototoxic and photoallergic potentials of a substance, and is adaptable to routine testing. In this protocol, the test substance is applied under occlusive patch using Freund's complete adjuvant during photoallergy induction to create an immunostimulation. Fluorescent lamps are used to irradiate the animals' skin. Histological examinations are also carried out to confirm the macroscopic reactions. Results obtained on reference substances are documented.