Approaches to labeling and identification of active site residues in glycosidases

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Abstract
Glycosidases play a key role in a number of biological processes and, as such, are of considerable clinical and bio‐technological importance. Knowledge of the identities of catalytically important active site residues is essential for understanding the catalytic mechanism, for enzyme classification, and for targeted bioengineering of glycosidases with altered characteristics. Here we review and discuss traditional strategies and novel approaches based on tandem mass spectrometry for the identification of the key active site residues in glycosidases.