Lanthanide-based resonance energy transfer
- 1 December 1996
- journal article
- Published by Institute of Electrical and Electronics Engineers (IEEE) in IEEE Journal of Selected Topics in Quantum Electronics
- Vol. 2 (4) , 1077-1087
- https://doi.org/10.1109/2944.577339
Abstract
Fluorescence resonance energy transfer is a powerful tool for studying nanometer-scale distances in biological macromolecules under physiological conditions. Using luminescent lanthanides instead of conventional fluorophores as donor molecules in energy transfer measurements offers many technical advantages and opens up a wide-range of new applications. Lanthanide photophysics and the instrumentation underlying these advantages are discussed. One new application, the study of conformational changes in the large protein complex actomyosin, which is responsible for muscle contraction and subcellular movement in many eucaryotes, is briefly discussed.Keywords
This publication has 37 references indexed in Scilit:
- Amine-Reactive Forms of a Luminescent Diethylenetriaminepentaacetic Acid Chelate of Terbium and Europium: Attachment to DNA and Energy Transfer MeasurementsBioconjugate Chemistry, 1997
- Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor.Proceedings of the National Academy of Sciences, 1996
- Luminescent Polyaminocarboxylate Chelates of Terbium and Europium: The Effect of Chelate StructureJournal of the American Chemical Society, 1995
- Tilting of the light-chain region of myosin during step length changes and active force generation in skeletal muscleNature, 1995
- Crossbridge tilting confirmedNature, 1995
- Use of fluorescent europium chelates as labels in microscopy allows glutaraldehyde fixation and permanent mounting and leads to reduced autofluorescence and good long‐term stabilityMicroscopy Research and Technique, 1994
- Streptavidin-based macromolecular complex labeled with a europium chelator suitable for time-resolved fluorescence immunoassay applicationsAnalytical Chemistry, 1990
- Fluorescence resonance energy transfer measurements of distances in actin and myosin. A critical evaluationJournal of Muscle Research and Cell Motility, 1987
- Time-Resolved Fluorescence of Lanthanide Probes and Applications in BiotechnologyCritical Reviews in Analytical Chemistry, 1987
- Monomolecular Layers and LightScientific American, 1970