Growth and Infectivity Titration of Virulent Infectious Bursal Disease Virus in Established Cell Lines from Lymphoid Leukosis Tumors

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Abstract
[Chicken] lymphoblastoid cell lines were investigated for susceptibility to virulent (wild) infectious bursal disease virus (IBDV) and attenuated IBDV by detection of fluorescent antigen in inoculated cells. LSCC-BK3 and LSCC-CU10 cells were susceptible to both types of IBDV; LSCC-1104-B-1 line was susceptible to only the attenuated IBDV. Two lymphoid leukosis and 2 Marek''s disease cell lines were refractory to growth of any type of IBDV. When the J1 strain of virulent IBDV was inoculated into LSCC-BK3 cells, intracellular and extracellular viruses began to increase logarithmically 4-8 h postinoculation and reached 106.3 and 107.3 median tissue culture infective dose/0.1 ml, respectively, at 72 h. Infectivity titration and serum-neutralization tests of virulent IBDV were possible with LSCC-BK3 cells.