Emerging methods for the production of homogeneous human glycoproteins
- 18 March 2008
- journal article
- review article
- Published by Springer Nature in Nature Chemical Biology
- Vol. 5 (4) , 206-215
- https://doi.org/10.1038/nchembio.148
Abstract
Most circulating human proteins exist as heterogeneously glycosylated variants (glycoforms) of an otherwise homogeneous polypeptide. Though glycan heterogeneity is most likely important to glycoprotein function, the preparation of homogeneous glycoforms is important both for the study of the consequences of glycosylation and for therapeutic purposes. This review details selected approaches to the production of homogeneous human N- and O-linked glycoproteins with human-type glycans. Particular emphasis is placed on recent developments in the engineering of glycosylation pathways within yeast and bacteria for in vivo production, and on the in vitro remodeling of glycoproteins by enzymatic means. The future of this field is very exciting.Keywords
This publication has 114 references indexed in Scilit:
- Glycosynthases Enable a Highly Efficient Chemoenzymatic Synthesis of N-Glycoproteins Carrying Intact Natural N-GlycansJournal of the American Chemical Society, 2009
- Expeditious Chemoenzymatic Synthesis of Homogeneous N-Glycoproteins Carrying Defined Oligosaccharide LigandsJournal of the American Chemical Society, 2008
- Glycoengineering of Human IgG1-Fc through Combined Yeast Expression and in Vitro Chemoenzymatic GlycosylationBiochemistry, 2008
- Not just for Eukarya anymore: protein glycosylation in Bacteria and ArchaeaCurrent Opinion in Structural Biology, 2008
- The Catalytic and Lectin Domains of UDP-GalNAc:Polypeptide α-N-Acetylgalactosaminyltransferase Function in Concert to Direct Glycosylation Site SelectionJournal of Biological Chemistry, 2008
- Synthesis and inhibitory activity of oligosaccharide thiazolines as a class of mechanism-based inhibitors for endo-β-N-acetylglucosaminidasesBioorganic & Medicinal Chemistry, 2008
- Chemoenzymatic synthesis of glycopeptides and glycoproteins through endoglycosidase-catalyzed transglycosylationCarbohydrate Research, 2008
- Recombinant human lactoferrin expressed in glycoengineered Pichia pastoris: effect of terminal N-acetylneuraminic acid on in vitro secondary humoral immune responseGlycoconjugate Journal, 2008
- Engineering of mucin-type human glycoproteins in yeast cellsProceedings of the National Academy of Sciences, 2008
- Substrate specificity of bacterial oligosaccharyltransferase suggests a common transfer mechanism for the bacterial and eukaryotic systemsProceedings of the National Academy of Sciences, 2006