THE CONVERSION OF PROGESTERONE TO 17α-HYDROXYPROGESTERONE BY HUMAN PLACENTA IN VITRO

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Abstract
The conversion of progesterone to 17α-hydroxyprogesterone by the soluble fraction of human placenta has been demonstrated in vitro. The incubation system contained the soluble supernatant fluid fraction of placental homogenate (105 000 × g), progesterone 4-14C as substrate, authentic 17α-hydroxyprogesterone as trap and a reduced triphosphopyridine nucleotide generating system as cofactor. The 17α-hydroxyprogesterone formed was isolated chromatographically and radiochemical purity was demonstrated by constant specific activity in a counter current distribution. Constant specific activity and radiochemical purity of the oxidation product and the acetylated derivative was also shown.