Analysis of Intact Tissue by Intermediate-Pressure MALDI on a Linear Ion Trap Mass Spectrometer
- 7 March 2006
- journal article
- editorial
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 78 (7) , 2465-2469
- https://doi.org/10.1021/ac0522761
Abstract
The use of an intermediate-pressure matrix-assisted laser desorption/ionization (IP-MALDI) source working at 0.17 Torr on a linear ion trap (LIT) was investigated for the analysis of tissue specimens, in particular, spinal cord sections. MALDI, with 2,5-dihydroxybenzoic acid (DHB) as the matrix, was employed for the detection of phospholipids. The matrix was applied to the tissue using electrospray to avoid analyte migration. The results indicate that analyzing tissue specimens at nontraditional MALDI vacuum pressures is possible. Coupling MALDI to an LIT permits the use of MSn, which is critical for the ability to identify compounds desorbed directly from tissue specimens. Using MSn, ions detected from m/z 600−1000 were characterized as phosphatidlycholines, PC. Specifically, using tandem MS, PC ions could be classified as either [M + H]+ or [M + Na]+ because the fragmentation patterns of protonated and sodiated phosphatidlycholines follow different pathways.Keywords
This publication has 20 references indexed in Scilit:
- Vitamin E Imaging and Localization in the Neuronal MembraneJournal of the American Chemical Society, 2005
- Direct profiling of the cerebellum by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry: A methodological study in postnatal and adult mouseJournal of Neuroscience Research, 2005
- Subcellular imaging mass spectrometry of brain tissueJournal of Mass Spectrometry, 2005
- Imaging Secondary Ion Mass Spectrometry of a Paint Cross Section Taken from an Early Netherlandish Painting by Rogier van der WeydenAnalytical Chemistry, 2004
- Integrating Histology and Imaging Mass SpectrometryAnalytical Chemistry, 2004
- Direct analysis of drug candidates in tissue by matrix‐assisted laser desorption/ionization mass spectrometryJournal of Mass Spectrometry, 2003
- Recent developments in atmospheric pressure MALDI mass spectrometryInternational Journal of Mass Spectrometry, 2002
- A high pressure matrix‐assisted laser desorption/ionization Fourier transform mass spectrometry ion source for thermal stabilization of labile biomoleculesRapid Communications in Mass Spectrometry, 2001
- Matrix‐assisted laser desorption/ionization Fourier transform mass spectrometric analysis of oxygenated triglycerides and phosphatidylcholines in egg tempera paint dosimeters used for environmental monitoring of museum display conditionsJournal of Mass Spectrometry, 2001
- Detection of pharmaceutical compounds in tissue by matrix-assisted laser desorption/ionization and laser desorption/chemical ionization tandem mass spectrometry with a quadrupole ion trapJournal of the American Society for Mass Spectrometry, 1999