Production of recombinant Von Willebrand factor by CHO cells cultured in macroporous microcarriers
- 1 September 1990
- journal article
- conference paper
- Published by Springer Nature in Cytotechnology
- Vol. 4 (2) , 163-171
- https://doi.org/10.1007/bf00365097
Abstract
Recombinant Chinese hamster ovary cells producing Von Willebrand factor have been successfully grown in gelatin macroporous microcarriers (Cultispher-G). Serum-free cultures were maintained in 1, 4, and 10 liter fermentors for more than two months. Comparative studies with Cytodex-3 microcarriers have been performed in 1 liter fermentors. The lower specific Von Willebrand factor productivity of CHO cells cultivated on Cultispher-G were offset by higher cell densities (107−2×107 cells/ml). Volumetric Von Willebrand factor productivity was influenced by oxygen concentration, and remained stable during scale-up from 1 to 10 liter fermentors.This publication has 10 references indexed in Scilit:
- Fiber bed reactor design for animal cell cultureBiotechnology & Bioengineering, 1989
- Fixed bed porous glass sphere (porosphere) bioreactors for animal cellsCytotechnology, 1988
- Growth of Anchorage–Dependent Cells on Macroporous MicrocarriersBio/Technology, 1986
- Industrial-Scale Production of Inactivated Poliovirus Vaccine Prepared by Culture of Vero Cells on MicrocarrierClinical Infectious Diseases, 1984
- High yields from microcarrier cultures by medium perfusionJournal of Cell Science, 1983
- Microcarrier technology. Present status and prospects.1983
- Cultivation of anchorage‐dependent cells and their applicationsJournal of Chemical Technology and Biotechnology, 1982
- The development and use of microcarrier and glass sphere culture techniques for the production of herpes simplex viruses.1981
- Growth of Cell-strains and Primary Cells on Micro-carriers in Homogeneous CultureNature, 1967
- A Glass Helix Perfusion Chamber for Massive Growth of Cells in vitro.Experimental Biology and Medicine, 1962