Polymeric cryoproteetants in the preservation of biological ultrastructure
- 2 August 1977
- journal article
- research article
- Published by Wiley in Journal of Microscopy
- Vol. 110 (3) , 223-238
- https://doi.org/10.1111/j.1365-2818.1977.tb00034.x
Abstract
The solid states formed by vitrified and frozen aqueous solutions of some hydrophilic polymers, able to act as biological cryoproteetants, have been studied by differential scanning calorimetry and freeze fracture electron microscopy. Glass transitions, devitrification, recrystallization and melting behaviour of aqueous solutions of polyvinylpyrrolidone, hydroxyethyl starch and dextran have been established. The vitrified polymer solutions exhibit a characteristic microspherical morphology which is not induced by the quench cooling process but is an inherent feature of the solutions themselves.This publication has 30 references indexed in Scilit:
- Polymeric cryoproteetants in the preservation of biological ultrastructureJournal of Microscopy, 1977
- Distribution of ions in a fluid-transporting epithelium determined by electron-probe X-ray microanalysisNature, 1976
- Aqueous glasses as matrices in freeze-fracture electron microscopyNature, 1976
- Storage of wild carrot cultures in the frozen stateCryobiology, 1974
- The use of polyvinylpyrrolidone as a cryoprotectant in the freezing of human lymphocytesCryobiology, 1974
- Conformation and hydration of sugars and related compounds in dilute aqueous solutionJournal of Solution Chemistry, 1973
- Low-temperature preservation of mammalian cells in tissue culture with polyvinylpyrrolidone (PVP), dextrans, and hydroxyethyl starch (HES)Cryobiology, 1972
- Human red cells under hypertonic conditions; A model system for investigating freezing damageCryobiology, 1972
- Studies on the molecular weight and cryoprotective properties of polyvinylpyrrolidone and dextran with bacteria and erythrocytesCryobiology, 1971
- Improved Cryofixation Applicable to Freeze EtchingProceedings of the National Academy of Sciences, 1971