AN EVALUATION OF A CHEMICAL METHOD FOR THE ESTIMATION OF OESTRIOL, OESTRONE AND OESTRADIOL-17β IN HUMAN URINE
- 1 November 1957
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 16 (1) , 41-48
- https://doi.org/10.1677/joe.0.0160041
Abstract
SUMMARY: The method described by Brown [1955] for the estimation of oestrone, oestradiol-17β and oestriol in urine has been critically investigated. 1. All checks on specificity so far carried out have confirmed that the method is specific for these oestrogens. 2. The method gives recoveries of 70–95%. 3. The precision of the method has been calculated for oestrogen levels between 0·0 and 40·0 μg/24 hr. From the figures obtained the fiducial ranges and percentage errors of determinations and the limits of sensitivity of the method have been calculated. 4. A number of substances which may occur in urine and which interfere in the method are listed.Keywords
This publication has 8 references indexed in Scilit:
- AN ADDITIONAL PURIFICATION STEP FOR A METHOD FOR ESTIMATING OESTRIOL, OESTRONE AND OESTRADIOL-17β IN HUMAN URINEJournal of Endocrinology, 1957
- 16α-Hydroxyoestrone in the urine of pregnant womenBiochemical Journal, 1957
- A method for the determination of oestriol, oestrone and oestradiol-17β in human urine by partition chromatography and colorimetric estimationBiochemical Journal, 1956
- Observations on the occurrence of 16-epioestriol in urineBiochemical Journal, 1956
- URINARY EXCRETION OF NATURAL OESTROGENS IN OOPHORECTOMIZED WOMEN TREATED WITH POLYOESTRADIOL PHOSPHATE (PEP)Acta Endocrinologica, 1956
- EXPERIMENTAL VERIFICATION OF THE ASSUMPTION UNDERLYING THE COLOUR CORRECTION EQUATION OF ALLENActa Endocrinologica, 1955
- A chemical method for the determination of oestriol, oestrone and oestradiol in human urineBiochemical Journal, 1955
- The isolation of 16-epioestriol from the urine of pregnant womenBiochemical Journal, 1955