Gas Channels for NH 3 : Proteins from Hyperthermophiles Complement an Escherichia coli Mutant
- 15 June 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 184 (12) , 3396-3400
- https://doi.org/10.1128/jb.184.12.3396-3400.2002
Abstract
Ammonium transport (Amt) proteins appear to be bidirectional channels for NH 3 . The amt genes of the hyperthermophiles Aquifex aeolicus and Methanococcus jannaschii complement enteric amtB mutants for growth at 25 nM NH 3 at 37°C. To our knowledge, Amt proteins are the first hyperthermophilic membrane transport proteins shown to be active in a mesophilic bacterium. Despite low expression levels, His-tagged Aquifex Amt could be purified by heating and nickel chelate affinity chromatography. It could be studied genetically in Escherichia coli .Keywords
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