Interaction of the MexA and MexB Components of the MexAB-OprM Multidrug Efflux System of Pseudomonas aeruginosa : Identification of MexA Extragenic Suppressors of a T578I Mutation in MexB
Open Access
- 1 October 2005
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 49 (10) , 4375-4378
- https://doi.org/10.1128/aac.49.10.4375-4378.2005
Abstract
A T578I mutation in MexB compromised the protein's contribution to antimicrobial resistance and negatively impacted its interaction with MexA. Mutations causing single amino acid changes in the C-terminal domain of MexA (R221H, L245F, E254K, and V259I) suppressed the antimicrobial susceptibility of a MexB T578I -expressing Pseudomonas aeruginosa strain and restored a MexA interaction with MexB T578I . These data confirm the importance of the MexA C-terminal region in MexB binding and the likely significance of the region surrounding T587I of MexB in MexA interaction.Keywords
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