Functional activity of oligosaccharide‐deficient (Na,K)ATPase expressed in Xenopus oocytes
- 26 September 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 238 (1) , 201-204
- https://doi.org/10.1016/0014-5793(88)80256-4
Abstract
(Na,K)ATPase from Torpedo californica was expressed in Xenopus laevis oocytes in the presence of tunicamycin by injecting mRNAs for the α- and β-subunits derived from the cloned cDNAs into the oocytes. The oligosaccharide-deficient ATPase thus synthesized was transported to the oocytes plasma membrane, where it exhibited virtually the same ATPase activity, ouabain-binding capacity and 86Rb+ transport activity as the fully glycosylated enzyme. We conclude that the oligosaccharide chains on the β-subunit has no effect on the catalytic activities of (Na,K)ATPase.Keywords
This publication has 25 references indexed in Scilit:
- Expression of functional (Na+ + K+)‐ATPase from cloned cDNAsFEBS Letters, 1987
- Molecular cloning and sequence analysis of the (Na+ + K+)-ATPase β subunit from dog kidneyBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1987
- Spatial relationship and conformational changes between the cardiac glycoside site and .beta.-subunit oligosaccharides in sodium plus potassium activated adenosinetriphosphataseBiochemistry, 1986
- Pig kidney Na+,K+‐ATPaseFEBS Letters, 1986
- Primary structure of the β‐subunit of Torpedo californica (Na+ + K+)‐ATPase deduced from the cDNA sequenceFEBS Letters, 1986
- Primary structure of the α-subunit of Torpedo californica (Na+ + K+)ATPase deduced from cDNA sequenceNature, 1985
- Mechanism of the Na+, K+ pump protein structure and conformations of the pureBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1982
- Molecular considerations relevant to the mechanism of active transportNature, 1981
- The Influence of Topology and Glycosylation on the Fate of Heterologous Secretory Proteins Made in Xenopus OocytesEuropean Journal of Biochemistry, 1981
- THE ENZYMATIC IODINATION OF THE RED CELL MEMBRANEThe Journal of cell biology, 1972