STUDIES ON BUD INDUCTION IN THE MOSS TREMA TODON BREVICALYX DIXON

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Abstract
Summary: Protonema of Trematodon brevicalyx remains bud‐free on basal medium (Knop's Major and Nitsch's Minor salts). Indoleacetic acid (IAA), gibberellic acid, abscisic acid, chelates, salicylic acid and altered temperature, pH, agar, sucrose levels, light levels and photoperiod do not induce buds but affect gemma initiation and growth rates of protonema. Buds are induced only by cytokinins, the largest number being produced with kinetin, followed by benzylaminopurine, bryokinin (2iP) and zeatin. When varying concentrations of IAA (10−4 to 10−8M) were added together with the optimum concentration of kinetin (10−5 M), normal shoot buds were formed but their number was considerably reduced.