Rapid Detection of Influenza A Pandemic (H1N1) 2009 Virus Neuraminidase Resistance Mutation H275Y by Real-Time Reverse Transcriptase PCR
- 1 May 2010
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 48 (5) , 1884-1887
- https://doi.org/10.1128/jcm.02540-09
Abstract
The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h.Keywords
This publication has 17 references indexed in Scilit:
- Detection of Molecular Markers of Drug Resistance in 2009 Pandemic Influenza A (H1N1) Viruses by PyrosequencingAntimicrobial Agents and Chemotherapy, 2010
- Oseltamivir Resistance and the H274Y Neuraminidase Mutation in Seasonal, Pandemic and Highly Pathogenic Influenza VirusesDrugs, 2009
- Oseltamivir-resistant pandemic (H1N1) 2009 influenza virus, October 2009.2009
- Influenza antiviral susceptibility monitoring activities in relation to national antiviral stockpiles in Europe during the winter 2006/2007 seasonEurosurveillance, 2007
- H5N1 Oseltamivir-resistance detection by real-time PCR using two high sensitivity labeled TaqMan probesJournal of Virological Methods, 2007
- Selection and persistence of non-nucleoside reverse transcriptase inhibitor-resistant HIV-1 in patients starting and stopping non-nucleoside therapyAIDS, 2006
- Isolation of drug-resistant H5N1 virusNature, 2005
- Molecular mechanisms of influenza virus resistance to neuraminidase inhibitorsVirus Research, 2004
- Detection of influenza virus resistance to neuraminidase inhibitors by an enzyme inhibition assayAntiviral Research, 2001
- Resistance of influenza viruses to neuraminidase inhibitors — a reviewAntiviral Research, 2000