CORTICOSTEROID SECRETION IN THE ADRENAL VENOUS BLOOD OF THE HYPOPHYSECTOMIZED DOG AS AN ASSAY FOR ACTH1

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Abstract
A method for the assay of ACTH was described. The secretion of 17-hydroxycortico-steroids in the adrenal vein of the hypophysectomized dog has a linear relation to the log of the dose following the injection of 1 to 10 milliunit quantities of ACTH into the dog''s peripheral circulation. It was shown that such animals may be used for the precise assay of purified preparations of ACTH. Dog blood ACTH content may also be determined without preliminary extraction of the ACTH from the blood sample. The method described appears to have the distinct advantage over other methods that the ACTH activity is determined directly in terms of cortico-steroids secreted by an individual dog''s adrenal cortex in response to repeated single doses of ACTH. The mean lambda value for 18 assays carried out by this procedure was found to be 0.118.
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