Pollen nuclei after ionising irradiation for egg transformation inNicotiana
- 1 September 1980
- journal article
- research article
- Published by Taylor & Francis in New Zealand Journal of Botany
- Vol. 18 (3) , 339-341
- https://doi.org/10.1080/0028825x.1980.10427250
Abstract
Pollen from Nicotiana alata and N. langsdorffii was gamma irradiated at doses previously used to treat donor pollen for egg transformation in these species. Observation with a light microscope of the second pollen mitosis showed that irradiation caused chromatin fragmentation, lack of metaphase orientation, and failure of the generative nucleus to divide.Keywords
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