Effects of Oxygen on Growth in Several Established Cell Lines2

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Abstract
Studies were made on the effects of various O2 concentrations on 4 established cell lines: strain L (NCTC 929), mouse liver epithelium (NCTC 1469), HeLa, and human skin (NCTC 1769). In 100 percent O2 some strain L cells survived up to 14 days; mouse liver cells, 9 days; HeLa cells, 3 days; human skin cells, 8 days. There was practically no proliferation in 100 percent O2, and all liver, HeLa, and skin cells died before termination of the experiment. In95 percent O2 + 5 percent CO2, the only significant proliferation was in the strain L cultures; all others decreased in number and finally died out. Of the 4 lines tested, human skin and HeLa cells appeared more sensitive to high O2 tensions than strain L or mouse liver cells. Cell proliferation in the presence of CO2 and 95 percent N2 was not so severely inhibited as that in 100 percent N2.