Cloning, sequencing and functional analysis of Magnaporthe grisea MVP1 gene, a hex-1 homolog encoding a putative ‘woronin body’ protein
Open Access
- 1 January 2004
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 230 (1) , 85-90
- https://doi.org/10.1016/s0378-1097(03)00858-9
Abstract
A hex-1 homolog named MVP1 was isolated from an appressoria cDNA library of the rice blast fungus Magnaporthe grisea. The transcript of ∼1.6 kb contains 546 bp of coding sequence with a 3′ untranslated region about 168 bp long and a 5′ untranslated region about 870 bp long. Southern gel blot analysis of genomic DNA following digestion with three restriction enzymes (BamHI, EcoRI, HindIII) indicated that the gene exists as a single copy in M. grisea genome. RNA gel blot analyses showed that MVP1 was highly expressed in germinating conidia and the mycelial stage compared to appressoria or non-germinated conidia. MVP1 showed a high degree of homology to the hex-1 gene recently described to encode a major protein in the woronin bodies of Neurospora crassa. Double homologous recombination was used to replace MVP1 with the hygR gene. MVP1 knockout mutants showed apical swellings when grown on agar plates containing 2% sorbose but they were not impaired in any other vegetative or pathogenic properties evaluated. The pathological and other phenotypic consequences of gene disruption are discussed.Keywords
This publication has 20 references indexed in Scilit:
- PDE1 Encodes a P-Type ATPase Involved in Appressorium-Mediated Plant Infection by the Rice Blast Fungus Magnaporthe griseaPlant Cell, 2001
- The adenylate cyclase gene MAC1 of Magnaporthe grisea controls appressorium formation and other aspects of growth and development.Plant Cell, 1997
- MAP kinase and cAMP signaling regulate infection structure formation and pathogenic growth in the rice blast fungus Magnaporthe grisea.Genes & Development, 1996
- The cAMP-dependent protein kinase catalytic subunit is required for appressorium formation and pathogenesis by the rice blast pathogen Magnaporthe grisea.Plant Cell, 1995
- Hydrophobicity of contact surface induces appressorium formation inMagnaporthe griseaFEMS Microbiology Letters, 1994
- Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea.Plant Cell, 1993
- cAMP Regulates Infection Structure Formation in the Plant Pathogenic Fungus Magnaporthe grisea.Plant Cell, 1993
- Molecular Genetic Analysis of the Rice Blast Fungus, Magnaporthe GriseaAnnual Review of Phytopathology, 1991
- DNA Fingerprinting with a Dispersed Repeated Sequence Resolves Pathotype Diversity in the Rice Blast Fungus.Plant Cell, 1991
- In vitro development of penetration structures in the rice blast fungus Magnaporthe griseaCanadian Journal of Botany, 1990