Primary structures of carp gonadotropin subunits deduced from cDNA nucleotide sequences

Publisher Website
Google Scholar
Abstract
The α and β subunits of carp gonadotropin (cGTH) were isolated by high performance liquid chromatography. They were identified to be the subunits of cGTH by bioassay and by partial N-terminal amino acid sequence analysis. To elucidate the complete primary structures of the α and β subunits of cGTH, cDNA cloning technique was employed. The α and β subunits consist of 95 and 115 amino acid residues, respectively. Homology of the α subunit of cGTH to those of mammalian GTH is around 70%. In comparison, the extent of homology of the β subunit between carp and salmon GTH (75%) is higher than that between fish and mammalian GTH (39–47%). Such comparative data suggest that the α subunit is highly conserved while the β subunit is diversified during the molecular evolution of vertebrate GTH.