MICRO-DISSECTION BY ULTRASONICATION AFTER PROLONGED OSO4 FIXATION - A TECHNIQUE FOR SCANNING ELECTRON-MICROSCOPY

  • 1 January 1982
    • journal article
    • research article
    • Vol. 14  (1) , 161-170
Abstract
A new method of tissue preparation for EM is described for the selective dissection of mammalian tissues. This technique, based on brittlization by prolonged osmication, dehydration to pure acetone and subsequent ultrasonication, was especially useful for the scanning electron microscopy visualization of microcirculation and basal laminas. Epithelial cells of treated tissues were largely fragmented and removed; collagen containing materials, notably epithelial and vascular basal laminas, withstood the vibratory insult. The extent of epithelium and basal lamina removal depended on tissue type and duration of ultrasonic treatment. Alone or in conjunction with specific enzymatic pretreatment, this new technique offers a method to better visualize known and unrecognized histological relationships. [Tissues were obtained from adult dogs (choroid plexus, motor cortex), guinea pigs (lung) and Long-Evans rats (jejunum and uterus).].

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