Establishment of a cell line of human skin squamous cell carcinoma in vitro

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Abstract
A cell line of a squamous cell carcinoma of human skin (HSC-I) has been established in vitro and successfully maintained proliferative in continuous tissue culture for about 2 1/2 yr since March 1978. Another cell line (HSC-Ib) has been established in vitro from the recurrence of the same cancer. It has been maintained for about 8 mo. since Dec. 1979. In the initial cultures in both cases 3T3 feeders were used. The cells grow in a monolayer in vitro and are epithelioid with anaplastic features. Chromosome analysis of HSC-I, frozen and thawed HSC-I, the transplanted tumor in a nude mouse and HSC-Ib all showed hypotetraploidy with the modal number 80, which reconfirms that HSC-I derives from the original skin tumor. The cell line is available to other investigators.