Characterization of nine polymorphic microsatellite loci in the fungus Botrytis cinerea (Ascomycota)
- 7 August 2002
- journal article
- research article
- Published by Wiley in Molecular Ecology Notes
- Vol. 2 (3) , 253-255
- https://doi.org/10.1046/j.1471-8286.2002.00207.x
Abstract
Nine microsatellite markers were characterized in the fungus Botrytis cinerea. Genomic DNA sequences from the partial sequencing of 12 000 bacterial artificial chromosome (BAC) clones, were screened by BLAST for various microsatellite motives, and primer pairs were designed. Cross‐amplification and polymorphism were assessed on 49 isolates from B. cinerea and two related species, collected from natural populations on several plants and locations.Keywords
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