Octopine, nopaline, and octopinic acid utilization in Pseudomonas

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Abstract
Culture media selective for Agrobacterium were inoculated with dilutions of soil and crown gall tumor suspensions. Colones on the selective media were replica plated on a medium with octopine or nopaline. More than 500 isolates were recovered, .apprx. 10% of which were confirmed as octopine-utilizing, fluorescent pseudomonads. These strains, together with 4 other strains of Pseudomonas that had been isolated in a previous study, were characterized for species identity, for utilization of various C sources and for capacity to grow with various opines and amino acids as the sole C and N source. The capacities for octopine and nopaline utilization were generally dissociated, which is similar to the situation found in Agrobacterium. Most of the octopine-utilizing strains of Pseudomonas showed markedly different growth kinetics in octopine and octopinic acid, 2 compounds that, in the Agrobacterium system, were classified into the same opine family. Generally, poor octopinic acid utilization was not correlated with poor ornithine utilization.