Determination of Total and Direct Bilirubin in Plasma by Means of a Bichromatic Method on a Centrifugal Analyser

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Abstract
A bichromatic method is described for the determination of both total and direct-reacting bilirubin using a miniature centrifugal analyser. Bilirubin was coupled with diazotised sulphanilic acid in strongly acidic reaction conditions, and sodium dodecyl sulphate was used as accelerator for the measurement of total bilirubin. The inclusion of dyphylline as a haem-binding agent greatly reduced interference, even in the presence of extreme haemolysis. The method was shown to be precise, to be more sensitive and less affected by haemolysis than alternative methods, and to allow the estimation of total and direct bilirubin within the same run.