Rapid Manual Immunoturbidimetric and Immunonephelometric Assays of Prealbumin, Albumin, IgG, IgA and IgM in Cerebrospinal Fluid

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Abstract
Manual turbidimetric kinetic assays (10-20 samples/h) for the determination of prealbumin, albumin, Ig[immunoglobulin]G and IgA in [human] CSF, and a turbidimetric end point assay for IgM were described for a mechanized microliter system (Eppendorf). It uses microcuvettes (total volume 300 .mu.l) and polyethylene glycol to increase sensitivity (detection limit, 1-3 mg/l) and reaction velocity (.ltoreq. min and 25 min, respectively). The results correlated satisfactorily with those obtained with laser end point nephelometry (1-2 h, total volume with Hyland laser 750 .mu.l, with Behring laser 225 .mu.l), e.g., correlation coefficient of > 0.9 for comparison of CSF samples, recovery rates .apprx. 90-110%, day to day precision < 10%. No interference with other proteins was observed (e.g., Hb). The turbidimetric assays were generally less expensive with respect to reagents (by factor 2- 20), and they required smaller sample volumes (by factor 2) and less technician time (by factor 2-4, especially for number of samples .ltoreq. 10) than both nephelometric end point assays, because they do not need daily established calibration curves. The turbidimetric kinetic assay in series to 6 samples can be compared to the Beckman immunochemistry analyzer which requires still less time, but is less sensitive and costlier.

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