The Separation of Human Globin Chains by Ion-Exchange chromatography on Cm-Sepharose Cl-6B
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Hemoglobin
- Vol. 3 (1) , 13-20
- https://doi.org/10.3109/03630267909069151
Abstract
A chromatographic procedure for the separation of human globin chains was described. This method used CM[carboxy methyl]-Sepharose CL-6B as ion-exchanger and NaCl gradients formed in sodium phosphate buffers containing urea and 0.05 M mercaptoethanol to elute the chains. One advantage of this system was that a column, once packed, may be used repeatedly for several chain separations, thus reducing cost and practical work. The resolution was also better than that on CM-cellulose using phosphate buffer gradients.Keywords
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