Dependence of Wound-induced Respiration in Potato Slices on the Time-restricted Actinomycin-sensitive Biosynthesis of Phospholipid
Open Access
- 1 July 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 60 (1) , 5-10
- https://doi.org/10.1104/pp.60.1.5
Abstract
Actinomycin D prevents the full development in a 24-hour period of both wound respiration and cyanide resistance only when given in the first 10 to 12 hours following the cutting of potato tuber (Solanum tuberosum var. Russet) slices. The capacity for choline incorporation into phosphatidylcholine increases with slice aging and is inhibited by actinomycin D in the same time-restricted way. The time-restricted effectiveness of actinomycin D applies to the cutting-elicited enhanced synthesis of three critical enzymes of phosphatidylcholine synthesis, namely phosphorylcholine-glyceride transferase, phosphorylcholine-cytidyl transferase, and phosphatidylphosphatase. By contrast, actinomycim D given at any time is without effect on the measurable levels after 24 hours of a selection of glycolytic and mitochondrial respiratory enzymes. Neither succinic dehydrogenase nor cytochrome oxidase activity increases with time in aging potato slices in the presence or absence of chloramphenicol. The foregoing observations emphasize the central role of phospholipid, and ultimately membrane biosynthesis, in the development of wound-induced respiration.This publication has 26 references indexed in Scilit:
- Inhibition of the Development of Induced Respiration and Cyanide-insensitive Respiration in Potato Tuber Slices by Cerulenin and DimethylaminoethanolPlant Physiology, 1977
- The Mechanism of Ethylene and Cyanide Action in Triggering the Rise in Respiration in Potato TubersPlant Physiology, 1975
- Potentiating effect of adenosine diphosphate in the uncoupling of oxidative phosphorylation in potato mitochondriaBiochemistry, 1973
- Enzymes of Phospholipid Metabolism in the Endoplasmic Reticulum of Castor Bean EndospermPlant Physiology, 1973
- Membrane Transformations in Aging Potato Tuber SlicesPlant Physiology, 1971
- Enzymic Deacylation of Lipids in Plants. The Effects of Free Fatty Acids on the Hydrolysis of Phospholipids by the Lipolytic Acyl Hydrolase of Potato TubersEuropean Journal of Biochemistry, 1971
- The Prevalence of Carbon-13 in Respiratory Carbon Dioxide As an Indicator of the Type of Endogenous SubstrateThe Journal of general physiology, 1970
- Phospholipid Synthesis in Aging Potato Tuber TissuePlant Physiology, 1968
- Ribosomal RNA Synthesis in Newly Sliced Discs of Potato TuberPlant Physiology, 1968
- The Onset of Tricarboxylic Acid Cycle Activity with Aging in Potato SlicesPlant Physiology, 1964