A Selective and Simple RP-HPLC Assay To Quantify Albendazole Metabolites in Plasma

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Abstract
A high performance liquid chromatographic (HPLC) method for the resolution of the two main metabolites of albendazole (ABZ): albendazole-sulphoxide (ABZ-SO) and albendazole-sulphone (ABZ-SO2) is proposed and compared to other methods previously reported. Results are presented for the linearity, reproducibility, and sensitivity of these two metabolites. This method is useful in pharmacokinetic studies of ABZ and ABZ-SO. One example of the possible application of this HPLC assay method in pharmacokinetic studies is provided. ABZ-SO and ABZ-SO2 were evaluated in plasma samples of mice after oral administration of a liquid formulation of ABZ-SO (50 mg/kg). During the 6 hours following oral administration to mice ABZ-SO concentrations were higher than ABZ-SO2. Cmax for ABZ-SO was achieved in less than 60 minutes after oral administration.