EFFECT OF SIMULTANEOUS THIAMIN AND RIBOFLAVIN DEFICIENCIES ON DETERMINATION OF TRANSKETOLASE AND GLUTATHIONE REDUCTASE

  • 1 January 1976
    • journal article
    • research article
    • Vol. 87  (5) , 741-748
Abstract
Thiamin and/or riboflavin status was assessed in rats simultaneously deprived of both vitamins and subsequently repleted with either one or the other in succession. Undernutrition in control rats depressed the activity of the erythrocyte enzymes (transketolase and glutathione reductase, respectively) used to assess status. The activity coefficients obtained by in vitro stimulation of the enzymes with their respective missing cofactors were not suppressed. The activity coefficients were superior indices of thiamin and riboflavin nutriture compared to the enzyme activities. When activity coefficients were used as the indices of vitamin status there did not appear to be interference from the simultaneous deficiency of the alternate vitamin. Apparent interference was encountered when the enzyme activity itself was used as the criteria.

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