Semipreparative Capillary Electrochromatography
- 29 March 2001
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 73 (9) , 1987-1992
- https://doi.org/10.1021/ac001302m
Abstract
Capillaries with inner diameters of 550 μm have successfully been packed with 1.5-μm octadecyl silica particles using frits made of macroporous polymers by the UV photopolymerization of a solution of glycidyl methacrylate and trimethylolpropane trimethacrylate. This type of frit is found superior to one made of low-melting point poly(styrene-co-divinylbenzene) beads. Bubble formation is not observed to occur within these capillary columns under our experimental conditions. Separations can be achieved with sample injection volumes as high as 1 μL. To demonstrate its semipreparative use, a mixture of 500 nL of taxol (20 mM) and its precursor, baccatin III (30 mM), is separated using such a column with a Tris buffer.Keywords
This publication has 22 references indexed in Scilit:
- Meetings: ConferencesAnalytical Chemistry, 2000
- Preparation and Characterization of Monolithic Porous Capillary Columns Loaded with Chromatographic ParticlesAnalytical Chemistry, 1998
- Macroporous Polyacrylamide/Poly(ethylene glycol) Matrixes as Stationary Phases in Capillary ElectrochromatographyAnalytical Chemistry, 1997
- Influence of sample plug width in capillary electrochromatographyJournal of Chromatography A, 1997
- Analysis of Corticosteroids in Biofluids by Capillary Electrochromatography with Gradient ElutionAnalytical Chemistry, 1997
- “Molded” Macroporous Poly(glycidyl methacrylate-co-trimethylolpropane trimethacrylate) Materials with Fine Controlled Porous Properties: Preparation of Monoliths Using Photoinitiated PolymerizationChemistry of Materials, 1997
- Column technology in capillary electrophoresis and capillary electrochromatographyElectrophoresis, 1997
- Preparation of Continuous Beds Derivatized with One-Step Alkyl and Sulfonate Groups for Capillary ElectrochromatographyAnalytical Chemistry, 1996
- Fritless Packed Columns for Capillary Electrochromatography: Separation of Uncharged Compounds on Hydrophobic HydrogelsAnalytical Chemistry, 1996
- Charged Polyacrylamide Gels for Capillary Electrochromatographic Separations of Uncharged, Low Molecular Weight CompoundsAnalytical Chemistry, 1995