A Laser Temperature-Jump Apparatus With Pulsed Spectrophotometric Detection Application To The Study Of Conformational Equilibria Of Adenyl 3′ 5′ Adenosine1
- 1 January 1977
- journal article
- research article
- Published by Taylor & Francis in Instrumentation Science & Technology
- Vol. 8 (1) , 1-16
- https://doi.org/10.1080/10739147708543434
Abstract
A Raman laser temperature-jump apparatus with highly sensitive (better than 10 −3 O.D. units) and very fast (rise time about 10 ns) pulsed spectrophotometric detection is described. A temperature jump of over 6°C is achieved within 20 ns in a special variable-path flow microcell. To illustrate the capabilities of the apparatus the kinetics of the conformation change of adenylyl 3‘→5’ adenosine in aqueous solution has been studied.Keywords
This publication has 9 references indexed in Scilit:
- Protein-folding dynamicsNature, 1976
- NMR studies of nucleic acids in solution. III. Conformational properties of adenylyl-3' →.5'-adenosine in aqueous solutionBiochemistry, 1976
- Stimulated Raman scattering from molecular hydrogen gas for fast heating in laser-temperature-jump chemical relaxation experimentsJournal of the American Chemical Society, 1975
- The Dynamics of Nucleic‐Acid Single‐Strand Conformation ChangesEuropean Journal of Biochemistry, 1973
- Laser Raman temperature-jump study of the kinetics of the triiodide equilibrium. Relaxation times in the 10-8 -10-7 second rangeJournal of the American Chemical Society, 1972
- Some Characteristics of a Pulsed Xenon Lamp for Use as a Light Source in Kinetic SpectrophotometryReview of Scientific Instruments, 1972
- Co-operative non-enzymatic base recognition III. Kinetics of the helix—coil transition of the oligoribouridylic · oligoriboadenylic acid system and of oligoriboadenylic acid alone at acidic pHJournal of Molecular Biology, 1971
- A Nanosecond Temperature-Jump ApparatusReview of Scientific Instruments, 1971
- Stimulated Raman effect. A new source of laser temperature-jump HeatingJournal of the American Chemical Society, 1970