An improved Giemsa N-banding technique for the identification of barley chromosomes

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Abstract
The combination of qualitative and quantitative techniques by combining acetocarmine and Glemsa staining demonstrated that karyotype analysis in barley could be conducted with greater precision than was previously possible. Early metaphase or prometa-phase chromosomes showed better banding pattern than the chromosomes at midmetaphase in somatic cells. The N-banding technique produces a diamond-shape centromertc region that has not been reported in earlier studies, and facilitates a better understanding of the Glemsa-banded karyotype in barley.