INDUCTION OF TUMOR RESISTANCE BY HYBRID CELL-LINES BETWEEN HUMAN ACUTE LYMPHOCYTIC-LEUKEMIA CELLS AND MOUSE MELOMA CELLS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 72  (5) , 705-712
Abstract
Three hybrid cell lines were derived from the fusion of BALB/c mouse NS-1 myeloma cells and human acute lymphocytic leukemia cells. Hybrid clones contained almost all of the mouse chromosomes and a few human chromosomes and induced tumors in BALB/c and BALB/c nu/nu mice when inoculated s.c. The tumors of BALB/c mice subsided spontaneously within 1 mo. The mice which survived the inoculation of 5-10 .times. 106 hybrid cells were able to reject a subsequent challenge with 5 .times. 106 NS-1 cells. Spleen cells or lymph node cells from immune mice were injected into BALB/c mice. These mice lived longer than the mice which received normal spleen cells or normal lymph node cells when challenged with 5 .times. 106 NS-1 cells. A rearrangement of tumor-specific antigens on human/mouse hybrid cells apparently can induce immunogenicity.