Strains of Schizosaccharomyces pombe with a disrupted swi1 gene still show some mating-type switching
- 1 December 1987
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 210 (3) , 485-489
- https://doi.org/10.1007/bf00327201
Abstract
The swi1 + gene is necessary for effective mating-type (MT) switching in Schizosaccharomyces pombe. It was cloned on a 4.2 kb genomic DNA fragment. By site-directed integration into the genome and gene disruption experiments it was proved that the swi1 + gene itself and not a suppressor had been isolated. Disruption of the swi1 + gene causes a phenotype identical to that of the original swi1 mutant, i.e. the strain still shows some MT switching. The swi1 gene is unique in the genome and gives rise to a 3 kb mRNA.Keywords
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