Intermediate stages in the metabolic conversion of benzpyrene to 8-hydroxy-benzpyrene in mice

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Abstract
Two compounds can be distinguished at intermediate stages in the metabolic conversion of benzpyrene to 8-hydroxybenz-pyrene in rabbits and mice; they have been purified by fluorescence chromatography of fresh extracts from various organs of animals treated with benzpyrene and are termed [BPX] and [BPF] by analogy with "BPX" and "BPF" present in the respective organs. Characteristics are given of the diffuse fluorescence bands of the organs before extraction of the purified ethanol extracts, of the absorption bands in ethanol, and of the fluorescence chromato-gram. [BPX] changes into [BPF] in vivo at the entrance of the small into the large intestine, and in vitro on keeping the organs for some days without a preservative like formol. The same change happens when the adsorbate of [BPX] on alumina is heated in vacuo to 100-200[degree]C. [BPX] is an as yet unknown benzpyrene derivative in combination with a cell constituent as carrier molecule; the similarity between the absorption spectra of [BPF] and 8-hydroxybenz-pyrene indicates that these are identical or closely related.